Puneet Souda (Pete) joined our laboratory in 2004 after attending graduate school at Purdue University in Indianapolis. He received his training in the laboratory of Dr Frank Witzmann and Dr. Bonnie Blazer-Yost. Previous to that he completed his Bachelors in 2001, also at Purdue University. Pete is involved in protein separations and Top Down mass spectrometry of the Human Salivary Proteome Project with Dr. Julian Whitelegge. In addition to that he is also part of the team developing mzML data format through the Human Proteome Organization Protein Standard Initiative. He also manages and oversees PMSL's computing resources including clusters and servers. Pete's expertise in large format 2D electrophoresis and 2D-LC are valuable for designing proteomics experiments involving both qualitative and quantitative measurements.
A partial listing of Puneet's publications:
Jonathan M Budzik, Luciano A Marraffini, Puneet Souda, Julian P Whitelegge, Kym F Faull, Olaf Schneewind. Amide bonds assemble pili on the surface of bacilli. Proceedings of the National Academy of Sciences (PNAS) USA. 2008 Jul 22;105(29):10215-20.
Haojie Lu, Chenggong Zong, Yueju Wang, Glen W Young, Ning Deng, Pete Souda, Xiaohai Li, Julian Whitelegge, Oliver Drews, Peng-Yuan Yang, Peipei Ping. Revealing the dynamics of 20S proteasome phosphoproteome: A combined CID and ETD approach. Molecular and Cellular Proteomics. 2008, Jun 25.
Paul Denny, Fred Hagen, Markus Hardt, Lujian Liao, Weihong Yan, Martha Arellanno, Sara Bassilian, Gurrinder Bedi, Pinmannee Boontheung, Daniel Cociorva, Claire Delahunty, Trish Denny, Jason Dunsmore, Kym Faull, Joyce Gilligan, Mireya Gonzalez-Begne, Frédéric Halgand, Steven Hall, Xuemei Han, Bradley Henson, Johannes Hewel, Shen Hu, Sherry Jeffrey, Jiang Jiang, Joseph Loo, Rachel Ogorzalek Loo, Daniel Malamud, James Melvin, Olga Miroshnychenko, Mahvash Navazesh, Richard Niles, Sung Park, Akraporn Prakobphol, Prasanna Ramachandran, Megan Richert, Sarah Robinson, Melissa Sondej, Puneet Souda, Mark Sullivan, Jona Takashima, Shawn Than, Jianghua Wang, Julian Whitelegge, H Witkowska, Lawrence Wolinsky, Yongming Xie, Tao Xu, Weixia Yu, Jimmy Ytterberg, David Wong, John Yates 3rd, Susan Fisher. The Proteomes of Human Parotid and Submandibular/Sublingual Gland Salivas Collected as the Ductal Secretions. Journal of Proteome Research. 2008 May;7(5):1994-2006.
Chris F Taylor, Norman W Paton, Kathryn S Lilley, Pierre-Alain Binz, Randall K Julian Jr, Andrew R Jones, Weimin Zhu, Rolf Apweiler, Ruedi Aebersold, Eric W Deutsch, Michael J Dunn, Albert J R Heck, Alexander Leitner, Marcus Macht, Matthias Mann, Lennart Martens, Thomas A Neubert, Scott D Patterson, Peipei Ping, Sean L Seymour, Puneet Souda, Akira Tsugita, Joel Vandekerckhove, Thomas M Vondriska, Julian P Whitelegge, Marc R Wilkins, Ioannnis Xenarios, John R Yates 3rd, Henning Hermjakob. The minimum information about a proteomics experiment (MIAPE). Nature Biotechnology. 2007 Aug; 25 (8):887-93
Jing Xie, Michael F Marusich, Puneet Souda, Julian Whitelegge, Roderick A Capaldi. The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS Lett. 2007 Jul 24;581
Julian Whitelegge, Frederic Halgand, Puneet Souda, Vlad Zabrouskov. Top-down mass spectrometry of integral membrane proteins. Expert Rev Proteomics. 2006 Dec ;3 (6):585-596
Qinxue Ding , Shoshanna Vaynman , Puneet Souda , Julian P Whitelegge , Fernando Gomez-Pinilla. Eur J Neurosci. Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysis. 2006 Sep ;24 (5):1265-76
Donald L Puppione , Lang M Yam , Sara Bassilian , Puneet Souda , Lawrence W Castellani , Verne N Schumaker , Julian P Whitelegge. Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications. Biochim Biophys Acta. 2006 Jun 18.
Julian P Whitelegge, Arthur Laganowsky, John Nishio, Puneet Souda, Huamin Zhang, William A Cramer. Sequencing covalent modifications of membrane proteins. J Exp Bot. 2006 Mar 30.
Blazer-Yost, B.L., P. Souda and F. Witzmann. Functional proteomics to assess qualitative and quantitative effects of steroid hormone binding. The Physiologist. 46:204, 2003
Book Chapters:
Puneet Souda, Frank Witzmann and Bonnie L. Blazer-Yost. Effect of Aldosterone on Na+ Transport in a Model Renal Epithelial Cell Line. Cell Volume and Signaling, V 559, Springer US, 2005.