Representative services
Protein identification
- Protein gel band identification
- Plasma/tissue – FASP or in-solution digest with stage-tip SCX and multiple fractions analyzed (typically 10)
- Post-translational modification (PTM) identification – as above with enrichment of phosphopeptides, acetyl-lys, dimethyl-arg, etc.
Protein / proteomics quantification
- Stable-isotope peptides and QQQ MS/MS-MRMs (Agilent Masshunter B.05; Skyline)
- Label-free using Progenesis LCMS for aligning and comparing chromatograms)
- Dimethyl labeling triplex stable isotope experiment with MaxQuant and Discoverer
Proteomics data analysis software
- MaxQuant 1.5.3.30
- Perseus 1.5.2.6
- Proteome Discoverer 1.4.1.2
- Mascot 2.4
Intact protein mass measurement, profiling, and quantification
- Low-resolution ESI typically LC-MS
- High-resolution ESI on FT-MS (Orbitrap, 7T LTQ-FT, 15T Bruker)
- Extracted Ion Chromatogram (EIC) method (low or high resolution)
Reverse-phase LC-MS with fraction collection
- LC-MS+ – Fractions reserved for top-down high-resolution FT-MS and other experiments
Top-down mass spectrometry
- High-resolution ESI on FT-MS (Orbitrap, 7T LTQ-FT, 15T Bruker) with collisionally-activated or electron capture/transfer dissociation (CAD and ECD/ETD; Prosight PC 3.0)
Immunocapture-MS
- Protein capture from complex fluids using immobilized antibody columns.
Native protein mass spectrometry
- Static nanospray on Orbitrap/LTQFT to 4000 m/z
- Nanospray or syringe pump infusion Bruker FTMS to 12,000 m/z
For protein or proteomics related inquiries, please contact Dr. Julian P. Whitelegge at jpw@chem.ucla.edu or (310) 794-5156
*Please allow up to 48 hours for a response
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